Anti-bacterial activity of ethanol extract of the stem of coffee parasite (Scurulla ferruginea (Robx. Ex Jack) Danser) and its secondary metabolite isolation

The aim of this study is to determine the antibacterial activity ethanol extract stem coffee parasite (Scurulla ferruginea (Robx. Ex Jack) Danser) using disc diffusion and microdilution methods. In study, secondary metabolites were isolated from stems Vacuum Liquid Chromatography Column continued by characterized compounds GC-MS (Gas chromatography-mass spectroscopy). Based on identification GC-MS, it was known that contains diisooctyl phthalate. results test method with an concentration 1% against Staphylococcus aureus, Salmonella typhi, Streptcoccus viridans, Escherichia coli, Streptococcus mutans, Bacillus aureus bacteria 6.35 mm, 6.7 8.95 7 8.75 mm 8.2 respectively. This result shows ability inhibit bacterial growth in medium category. MIC values for 2500 g/mL, while viridans 5000 g/mL. MBC value greater than only coli can be killed at a g/mL typhi showed Scurulla as inhibitor.